Types of Eosin Dye: Classifications and Applications
A dye is an organic substance that can impart color to various substrates after it is absorbed into them. Dyes can be natural or synthetic. Chemical bonds are formed between dye molecules and surfaces, causing the dye molecules to become a part of the material. In this article, we will discuss Eosin dye and its applications in different industrial applications.
Xanthene is a class of dyes divided into three groups called subgroups and each subgroup has its subgroup name. The use of dyes is widespread in a comprehensive range of applications, including food, pharmaceutical, cosmetics, plastics, textile, inks, paint, leather, and paper industries. Eosin is an acidic orange-pink dye that provides very strong staining properties.
Hematoxylin and Eosin Y (H and E) are one of the most widely used techniques in histopathology laboratories. If are you still wondering what is Eosin dye and understand the synthetic application of Eosin Y in a variety of industries, continue reading this guide further.
What is Eosin Dye?
Eosin is a fluorescent dye, a xanthene dye also known as red dye produced with the reaction between bromine and fluoresce- which further ties up to salts with eosinophilic compounds that have positive charges. Typically, H&E staining uses this stain as a counterstain for hematoxylin.
The histological examination of cytoplasm, red blood cells, collagen, and muscle fibers can be performed with eosin. Furthermore, they are most commonly used as photosensitizers in organic synthesis.
Types of Eosin Dye
Eosin dye is a bromine derivative of fluorescein, which is classified into two very closely related dyes; namely Eosin Y (Eosin yellowish) and Eosin B (Eosin bluish). Let’s know more about them in detail.
1. Eosin Y:
Eosin Y is a derivative of tetrabromo, while Eosin B is a derivative of fluorescein called dibromo dinitro. Eosin Y is also called disodium 2-(2, 4, 5, 7-tetrabromo-6-oxido-3-oxo-3H-xanthen-9-yl) benzoate. The chemical formula of EOSIN Y is C20H6Br4Na2O5, while its molar mass is 691.85.
In the fluorochrome molecule, fluorochrome exists in two forms; one is the quinoid form, which is a colored and fluorescent form of fluorescein, while the other is a colorless and nonfluorescent lactone form. In its free acid form (quinoid form), the carboxylic group (free form) of Eosin Y can readily be converted or transformed into quinones in equilibrium with lactones.
Furthermore, the dissociation of hydroxyl or carboxylic groups may lead to the ionization of eosin Y in a weakly acidic medium.
2. Eosin B:
Eosin B is a type of eosin that is also known as a dye compound. The other form of eosin B dye is 4, 5-dibromo-2, 7dinitro-fluorescein, also known as 4′, 5′-dibromo-2′, 7′-dinitro-3-oxo-3Hspiro[2-benzofuran-1,9′-xanthene]-3′,6′ diolate.
The staining of cytoplasm and red blood cells with eosin is commonly used in histology, particularly in conjunction with the staining of collagen and muscle fibers with hematoxylin.
Use of Eosin Dye in Different Industrial Applications
Eosin dye is widely used in a comprehensive range of industrial applications, including drugs, cosmetics, foods, pigments, textiles, pencil lead, paper inks, color filters, photographic materials, laundry detergent, varnish, liquid crystal displays, and more. The use of Eosin as a fluorescent indicator was carried out in acid-base titration for the measurement of vitamins (B1 and B2) in food.
As it works as an important radioactive tracer, it can also be used in different drugs and medicines in the pharmaceutical industry. In addition, the use of Eosin is also popular as a photosensitizer in organic synthesis.
Application of Eosin Y in Drug Analysis
The analysis of pharmaceutical compounds has been successfully performed using several commercially available xanthene dyes. Spectrophotometric or spectrofluorimetric methods have been widely used to determine the presence of several basic drugs using Eosin Y, an acidic dye belonging to the xanthene group of dyes.
Conclusion
In conclusion, it can be said that the use of Eosin or Eosin dye is widespread in various industries, including food, drugs, cosmetics, textiles, plastics, paints, ink, laundry detergent, color filters, varnish, and photographic materials. If you are an industrialist in need of quality Eosin dye for your industrial applications, Hridhan Chem Pvt Ltd can be an ideal choice for your business needs.
Hridhan Chem is a reputed manufacturer and exporter of premium quality Eosin dyes in India, delivering high-end products to its worldwide customers. The company follows all international regulations and guidelines to ensure safety and high standards.
Interested in buying top-rated Eosin red dye or need more info on a variety of synthetic dyes or pigments we produce at our manufacturing facility, please contact us today or visit our website!
