Types of Eosin Dye: Classifications and Applications
A dye is an organic substance that imparts color to various substrates after it is absorbed into them. Dyes can be natural or synthetic. Chemical bonds are formed between dye molecules and surfaces, causing the dye molecules to become part of the material. This article will discuss Eosin dye and its various industrial applications.
Xanthene is a class of dyes divided into three groups, called subgroups, each with its own subgroup name. Dyes are widely used in a comprehensive range of applications, including the food, pharmaceutical, cosmetics, plastics, textile, inks, paint, leather, and paper industries.
For instance, synthetic dyes used for food coloring play a significant role in the food industry. Eosin is an acidic orange-pink dye that provides very strong staining properties. Colorants are also vital in the plastics industry, as they are widely employed to enhance the visual appeal of products. You can learn more about different types of plastic colorants and their uses in various applications.
Hematoxylin and Eosin Y (H and E) are two of the most widely used techniques in histopathology laboratories. If you are still wondering what Eosin dye is and understand the synthetic application of Eosin Y in various industries, continue reading this guide.
What is Eosin Dye?
Eosin is a fluorescent xanthene dye, also known as red dye, produced by the reaction between bromine and fluorescein. It further binds to salts with eosinophilic compounds that have positive charges. Typically, H&E staining uses this stain as a counterstain for hematoxylin.
Eosin can be used to histologically examine cytoplasm, red blood cells, collagen, and muscle fibers. Furthermore, it is most commonly used as a photosensitizer in organic synthesis.
Read more: Classification of phloxine-b
Types of Eosin Dye
Eosin dye is a bromine derivative of fluorescein, which is classified into two very closely related dyes, namely Eosin Y (Eosin yellowish) and Eosin B (Eosin bluish). Let’s know more about them in detail.
1. Eosin Y:
Eosin Y is a derivative of tetrabromophenanthrene, while Eosin B is a derivative of fluorescein called dibromodinitro. Eosin Y is also called disodium 2-(2, 4, 5, 7-tetrabromo-6-oxido-3-oxo-3H-xanthen-9-yl) benzoate. The chemical formula of EOSIN Y is C20H6Br4Na2O5, while its molar mass is 691.85.
In the fluorochrome molecule, fluorochrome exists in two forms: the quinoid form, a colored and fluorescent form of fluorescein, while the other is a colorless and nonfluorescent lactone form. In its free acid form (quinoid form), the carboxylic group (free form) of Eosin Y can readily be converted or transformed into quinones in equilibrium with lactones.
Furthermore, the dissociation of hydroxyl or carboxylic groups may lead to the ionization of eosin Y in a weakly acidic medium.
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2. Eosin B:
Eosin B is a type of eosin that is also known as a dye compound. The other form of eosin B dye is 4, 5-dibromo-2, 7dinitro-fluorescein, also known as 4′, 5′-dibromo-2′, 7′-dinitro-3-oxo-3Hspiro[2-benzofuran-1,9′-xanthene]-3′,6′ diolate.
Eosin staining of cytoplasm and red blood cells is commonly used in histology, particularly in conjunction with hematoxylin staining of collagen and muscle fibers.
Use of Eosin Dye in Different Industrial Applications
Eosin dye is widely used in various industrial applications, including drugs, cosmetics, foods, pigments, textiles, pencil lead, paper inks, color filters, photographic materials, laundry detergent, varnish, liquid crystal displays, and more. The use of Eosin as a fluorescent indicator was carried out in acid-base titration for the measurement of vitamins (B1 and B2) in food.
As an important radioactive tracer, eosin can also be used in different drugs and medicines in the pharmaceutical industry. In addition, it is also popular as a photosensitizer in organic synthesis.
Read More: What are Dyes
Application of Eosin Y in Drug Analysis
Several commercially available xanthene dyes have been successfully used to analyze pharmaceutical compounds. Spectrophotometric or spectrofluorimetric methods have been widely used to determine the presence of several basic drugs using Eosin Y, an acidic dye belonging to the xanthene group of dyes.
Conclusion
In conclusion, Eosin or Eosin dye is widespread in various industries, including food, drugs, cosmetics, textiles, plastics, paints, ink, laundry detergent, color filters, varnish, and photographic materials. If you are an industrialist needing quality Eosin dye for your industrial applications, Hridhan Chem Pvt Ltd can be an ideal choice for your business needs.
Hridhan Chem is a reputed manufacturer and exporter of premium-quality Eosin dyes in India. The company delivers high-end products to its worldwide customers. It follows all international regulations and guidelines to ensure safety and high standards.
If you are interested in buying top-rated Eosin red dye or need more information on a variety of synthetic dyes or pigments we produce at our manufacturing facility, please contact us today or visit our website!
