What is Phloxine B and its Use in different countries?
Phloxine B is a red dye used to color drugs and cosmetics in the United States as well as coloring foods in Japan. Phloxine B has several other applications, including its ability to kill bacteria, dye cell viability, and stain biological samples. Red tones are produced in the cytoplasm and connective tissue of cells after staining with hematoxylin-phloxine-saffron (HPS).
Phloxine B acts bactericidal against methicillin-resistant Staphylococcus aureus (MRSA) and several other gram-positive bacteria in the presence of light. As long as a dose of 1.25 mg/kg per day is taken, phloxine B is deemed to be safe for humans by the Food and Drug Administration. Well! In this article, you will learn what is Phloxine B, and its uses in different countries.
So, let’s get into it!
What is Phloxine B?
Phloxine B also called phloxine refers to a water-soluble red dye used to impart colors to cosmetics and drugs in the United States. It is used as a food coloring in Japan. The carboxyphenyl ring contains four chlorine atoms and four bromine atoms at the positions of 2, 4, 5, and 7, which distinguish it from fluorescein. There is a maximum absorption wavelength of 540 nm and a maximum emission wavelength of 564 nm for this type of red dye.
As a color additive for food, drugs, and cosmetics, Phloxine B is a red dye of the USP grade. Furthermore, it has been used to distinguish Gram-positive bacteria from Gram-negative bacteria instead of Gram staining. This antibiotic inhibits methicillin-resistant bacteria at 100 µg/ml. There is also evidence that phloxine B modulates the activity of wild-type CFTR (Ki = 38 M) and mutant delta F508 CFTR (Ki = 33 M).4
The Use of Phloxine B
Phloxine B as a red coloring agent is widely used in pharmaceutical and cosmetics as well as coloring food items based on their approved use in different countries. It has several other applications besides industrial applications, including antimicrobial properties, viability dyes, and biological stains. The cytoplasm and connective tissue are stained with hematoxylin-phloxine-saffron (HPS) to create a red color.
They are types of acid dyes that are anionic and most commonly used in the textile industry for coloring different types of natural fibers, including silk, wool, cotton, and synthetics such as Rayon, acrylic, polyester, Paints, inks, plastics, and leather are among the fields where they are used less extensively.
In addition, it is used with Azure A for stains that look at nuclei, cell granules, and micro-organisms. It is a counterstain for collagen, following Mayer's Hemalum. The anterior pituitary cells can be stained differently for differential staining.
PRODUCT SPECIFICATIONS
Chemical Name:
Fluorescein, 2',4',5',7'-tetrabromo-4,5,6,7-tetrachloro-, disodium salt
Synonyms:
- Aizen Acid Phloxine PB
- C.I. 45410
- Cyanosin
- D&C Red No. 28
- Eosin Blue
- Eosine Blue
- Eosine bluish
- Food Red No. 104
- Food Dye Red No. 104
- Red No. 104
- Acid Red 92
- Orient Water Pink 2
- Japan Red 104
- Phloxin B
- Phloxine P
- 11969, Red
- 3427 Veri Pur Pink
CAS Number:
18472-87-2
EC number:
242-355-6
Concentration:
95% - 100%
RTECS Number:
LM5900000
Colour Index Number:
45410
Chemical Name:
Fluorescein, 2',4',5',7'-tetrabromo-4,5,6,7-tetrachloro-, disodium salt
Formula: C₂₀H₂Br₄Cl₄Na₂O₅
Variant Qualities of Phloxine B Hridhan Chem manufactures
We at Hridhan Chem Pvt Ltd manufacture 3-4 qualities of this product, including;
- Crude
- USFDA Approved Extra Pure
- Salt-Free
- Lake
How safe Phloxine B is?
Since there is no animal or human evidence to support the category of "harmful by ingestion," Phloxine B has been classified to be safe for use on humans. While unintentional ingestions are not considered hazardous in occupational settings, they are not generally recommended. However, it can cause some kind of eye irritation or damage in rare people, but it is very low in some cases. When the material enters the skin without causing harm, wounds, lesions, or abrasions can still cause health problems.
Talking about animal models, there are no signs of adverse health effects or respiratory tract irritation with this material. The best hygiene practices call for minimizing occupational exposure and using appropriate control measures. The inhalation of excess particulate matter may exacerbate respiratory impairment, airway disease, and chronic lung conditions like emphysema and chronic bronchitis. But it will happen only when not taken in excessive amounts or not consumed properly as per the given dosage.
Conclusion
Phloxin B (C.I. 45410) is used as the dye for preparing Certistain, the dry solution for cytoplasmic staining in histological sections of human origin. The majority of histological laboratories perform Hemytoxylin and Eosin (H&E) overview staining with it. Combined with Eosin and Phloxine, the dyes generate a greater range of colors in the tissue because both bind positively to plasma proteins. The dye is chemically certified and approved in accordance with strict specifications at the highest quality level and based in different countries. Due to its IVD status and CE registration, it can be used hassle-free when conducting diagnostics or obtaining accreditation for laboratories.
If you are a business looking for premium quality Phloxine B red dye for your industrial uses, Hridhan Chem is an ideal choice for your business. Hridhan is a reputed manufacturer and exporter of quality Phloxine B with a high degree of purity that is 100% safe and can be used for different purposes in different industrial applications. In addition, if you need more information on the different types of dyes we produce at our company, please visit our website or directly contact us today!
